%0 Journal Article
%T Extracorporeal photochemotherapy induces bona fide immunogenic cell death
%A Alp Yurter
%A Douglas Hanlon
%A Eve Robinson
%A Felix Rivera-Molina
%A Kazuki Tatsuno
%A Lorenzo Galluzzi
%A Najla Arshad
%A Olga Sobolev
%A Patrick Han
%A Richard L. Edelson
%A Takahiro Yamazaki
%J Archive of "Cell Death & Disease".
%D 2019
%R 10.1038/s41419-019-1819-3
%X a Residual number of B16-OVA cells upon exposure to 8-methoxypsoralen (8-MOP) in the context of UVA light at the indicated dose, 4J UVA irradiation only, or the indicated dose of cisplatin (CDDP) for 48£¿h, or upon exposure to repeated freeze/thawing (F/T) and culture in control conditions for 48£¿h. Representative micrographs and quantitative data are reported. n£¿=£¿3; **p£¿<£¿0.01, ***p£¿<£¿0.001 (Student¡¯s t-test), as compared to cells receiving 4J UVA light only. b Percentage of AnnV+PI£¿ and AnnV+PI+ B16-OVA cells upon exposure to 8-MOP plus 4J UVA light (8-MOPA) and culture in control conditions for the indicated time. Representative dotplots with percentages of events in relevant quadrants, and quantitative results are reported. n£¿=£¿3; *p£¿<£¿0.05, ***p£¿<£¿0.001 (Student¡¯s t-test), as compared to untreated cells. c Residual clonogenic potential of B16-OVA cells optionally exposed to 8-MOP in the context of no irradiation or UVA light at the indicated dose. Representative images and quantitative results are reported. n£¿=£¿3; ***p£¿<£¿0.001 (Student¡¯s t-test), as compared to cells exposed to the same UVA dose in the absence of 8-MOP. d Percentage of C57BL/6 mice developing tumors upon subcutaneous inoculation of B16-OVA cells exposed in vitro to the indicated UVA dose in the presence or absence of 8-MOP. Quantitative results from one representative experiment involving 10 mice per group are reporte
%K Tumour immunology
%K Cell death
%K Immune cell death
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6675789/