Inhibition of the BET family reduces its new target gene IDO1 expression and the production of l-kynurenine

a The number of the significantly differentially expressed genes (fold change？>？2, p？<？0.05) in Ty-82 cells treated with 1？μM compound 9 for 12 or 24？h. b Genes of the differentially downregulated expression in the compound 9-treated Ty-82 cells were plotted as the Venn diagram to display the commonly downregulated genes. c Heatmaps of 70 common genes of the differentially downregulated expression in Ty-82 cells treated with compound 9 for 0, 12, or 24？h, ranked (1–70) by fold change from big to small after 24-h treatments. Also see Supplementary Table S1–3. d–g Ty-82 cells were treated with the BET inhibitor ABBV-075 at the indicated concentrations for the indicated time. The mRNA (d, e) and protein (f, g) levels of IDO1 were determined by RT-qPCR and western blotting, respectively. Data were from three independent experiments, and if applicable, were expressed as mean？±？SD (error bar); *p？<？0.05; **p < 0.01; ***p < 0.00