The natural compound neobractatin inhibits tumor metastasis by upregulating the RNA-binding-protein MBNL2

a Chemical structure of NBT. b Cell viability assays. The MTT assay was used to examine the cell viability in the absence or presence of different doses of NBT for 24？h. c Wound healing assay. A scratch was made in monolayers of MDA-MB-231 and A549 cells, and the migration ability of the cells treated with or without different concentrations of NBT was monitored with an inverted microscope. d Cell migration measured by transwell assays. The cells were treated with different concentrations of NBT for 24？h, and migrated or invaded cells were fixed and stained with 0.1% crystal violet. e The summary of data for transwell migration assays. f Western blot asssay. MDA-MB-231 and A549 cells were treated with NBT (2.5？μM) for indicated time points. Data shown are mean？±？SD of three independent experiments. ***P？<？0.00