Hypoxia inducible factor 1α in vascular smooth muscle cells promotes angiotensin II-induced vascular remodeling via activation of CCL7-mediated macrophage recruitment

WT mice were infused with saline or 1000？ng/kg/min Ang II for 28 days. a Immunofluorescence analysis of representative cross-sections of mice aortas for HIF1α (red) and α-SMA (green), nuclei was stained with DAPI. VSMCs were isolated form WT mice and treated with 1？μM Ang II for 24？h. b Hif1a mRNA was measured by qPCR analysis. c HIF1α protein was detected by western blot. **P？<？0.01, ***P？<？0.001, n？=？3 per group, statistical significance was determined by the unpaired t-tes