Altered T Cell Migratory Capacity in the Progression from Barrett Oesophagus to Oesophageal Adenocarcinoma

Significantly lower frequencies of circulating CXCR3+and CCR6+T cells in OAC patients while CCR3+T cells are significantly higher in the circulation of Barrett Oesophagus and OAC patients. Whole blood from healthy control (Healthy), oesophagitis, Barrett’s and OAC and oesophageal biopsies from normal tissue adjacent to Barrett Oesophagus tissue (Normal), oesophagitis, Barrett’s and OAC patients were immunophenotyped by flow cytometry by staining for CD3, CD4, CD8, CXCR3, CCR5, CCR4, CCR3 and CCR6. Scatter plots showing percentage frequencies of CD4+ or CD8+ T cells expressing CCR3, CCR4, CCR5, CCR6 and CXCR3 in healthy control (Healthy), oesophagitis, Barrett’s and OAC patient blood (Blood) and normal tissue adjacent to Barrett Oesophagus tissue (Normal), oesophagitis, Barrett’s and OAC patient tissue (Tissue). All data is presented as mean？±？SEM, *p？≤？0.05, **p？≤？0.01, ***p？≤？0.001, T test (Mann Whitney), (Blood: Normal？=？7–8, Oesophagitis？=？3–4, Barrett’s？=？7–8, OAC？=？4–5, Tissue: Normal？=？4–6, Oesophagitis？=？3, Barrett’s？=？4, OAC？=？4