Long noncoding RNA PCAT1, a novel serum-based biomarker, enhances cell growth by sponging miR-326 in oesophageal squamous cell carcinoma

a PCAT1 expression in 39 paired ESCC samples and adjacent normal tissues determined by RT-qPCR. β-actin was used as an internal reference. Statistical significance was assessed using the paired Student’s t test. ***P？<？0.001. b Analysis of PCAT1 expression in ESCA (TCGA’s abbreviation for oesophageal carcinoma) tissues and normal tissues by using the TCGA database. Statistical significance was assessed using the Mann-Whitney test. **P？<？0.01. c The expression of PCAT1 was detected in eight ESCC cell lines and two immortalized normal oesophageal epithelial cell lines (NE2 and NE3) by RT-qPCR. GAPDH was used as an internal reference. The expression of PCAT1 in NE2 cells was defined as 1. d RT-qPCR analysis of PCAT1 half-life in KYSE30 cells treated with actinomycin D. GAPDH was used as an internal referenc