Knockdown of TRIM65 inhibits autophagy and cisplatin resistance in A549/DDP cells by regulating miR-138-5p/ATG7

a mRNA expression of TRIM family proteins in A549/DDP and A549 cells. *P？<？0.05, **P？<？0.01, ***P？<？0.001, ****P？<？0.0001 versus A549 cells. b Protein expression of TRIM65 in A549/DDP and A549 cells. c Protein expression of TRIM65 in A549/DDP cells transduced with TRIM65 shRNAs (shTRIM65–1, shTRIM65–2, and shTRIM65–3) or control shRNA (shNC). d–f A549/DDP cells transduced with shTRIM65–1, shTRIM65–2 or shNC for 24？h, and then treated with 0, 10, and 20？μM cisplatin for 24？h. Annexin V-PI staining d, e and caspase3 activity test f were performed to assess early apoptosis. ***P？<？0.001 versus shNC and treated with the same concentration of cisplatin; ###P？<？0.001 versus shNC？+？0？μM cisplatin. g Protein expression of TRIM65 in A549 cells transduced with TRIM65 overexpressing (TRIM65 OE) or Vector virus. h–j A549 cells transduced with TRIM65 OE or Vector for 24？h, and then treated with 0, 10, and 20？μM cisplatin for 24？h. Annexin V-PI staining (d, e) and caspase3 activity test (f) were performed to assess early apoptosis. ***P？<？0.001 versus Vector and treated with the same concentration of cisplatin; ###P？<？0.001 versus Vector？+？0？μM cisplati