%0 Journal Article
%T Knockdown of TRIM65 inhibits autophagy and cisplatin resistance in A549/DDP cells by regulating miR-138-5p/ATG7
%A Jicheng Tantai
%A Xufeng Pan
%A Yong Chen
%A Yuzhou Shen
%J Archive of "Cell Death & Disease".
%D 2019
%R 10.1038/s41419-019-1660-8
%X a mRNA expression of TRIM family proteins in A549/DDP and A549 cells. *Pˋ<ˋ0.05, **Pˋ<ˋ0.01, ***Pˋ<ˋ0.001, ****Pˋ<ˋ0.0001 versus A549 cells. b Protein expression of TRIM65 in A549/DDP and A549 cells. c Protein expression of TRIM65 in A549/DDP cells transduced with TRIM65 shRNAs (shTRIM65每1, shTRIM65每2, and shTRIM65每3) or control shRNA (shNC). d每f A549/DDP cells transduced with shTRIM65每1, shTRIM65每2 or shNC for 24ˋh, and then treated with 0, 10, and 20ˋ米M cisplatin for 24ˋh. Annexin V-PI staining d, e and caspase3 activity test f were performed to assess early apoptosis. ***Pˋ<ˋ0.001 versus shNC and treated with the same concentration of cisplatin; ###Pˋ<ˋ0.001 versus shNCˋ+ˋ0ˋ米M cisplatin. g Protein expression of TRIM65 in A549 cells transduced with TRIM65 overexpressing (TRIM65 OE) or Vector virus. h每j A549 cells transduced with TRIM65 OE or Vector for 24ˋh, and then treated with 0, 10, and 20ˋ米M cisplatin for 24ˋh. Annexin V-PI staining (d, e) and caspase3 activity test (f) were performed to assess early apoptosis. ***Pˋ<ˋ0.001 versus Vector and treated with the same concentration of cisplatin; ###Pˋ<ˋ0.001 versus Vectorˋ+ˋ0ˋ米M cisplati
%K Lung cancer
%K Cell biology
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6546683/