NETO2 promotes invasion and metastasis of gastric cancer cells via activation of PI3K/Akt/NF-κB/Snail axis and predicts outcome of the patients

a Representative immunohistochemical staining (IHC) images of NETO2 in adjacent normal tissue, gastric cancer tissues with different invasion depth and metastatic lymph node. Scale bar？=？50？μm. b The IHC score of NETO2 in gastric cancer tissues was significantly higher than that in adjacent normal tissues. c High expression of NETO2 was more frequent in gastric cancer tissues than in adjacent normal tissues. d Data in GEO {"type":"entrez-geo","attrs":{"text":"GSE29272","term_id":"29272"}}GSE29272 showed higher mRNA level of NETO2 in gastric cancer tissues compared to adjacent normal tissues. e Data in TCGA database showed higher mRNA level of NETO2 in gastric cancer tissues compared to adjacent normal tissues. f mRNA levels of NETO2 were higher in 20 fresh surgical gastric tumor specimens (t) than that in paired adjacent normal tissues (n). g NETO2 protein levels were higher in eight fresh surgical gastric cancer (t) tissues than that in adjacent normal (n) tissues. h Kaplan–Meier estimation indicated significantly lower overall and disease-free survival rates in patients with NETO2high gastric cancer than that in NETO2low patients. i Data in KMPLOT database showed that NETO2high patients had significantly lower overall and disease-free survival rates than that in NETO2low patients. j Data in TCGA database showed that NETO2high patients had significantly lower overall survival rate than that in NETO2low patients. *p？<？0.05, ***p？<？0.00