Hemi-methylated CpG sites connect Dnmt1-knockdown-induced and Tet1-induced DNA demethylation during somatic cell reprogramming

a Schematic illustration of the three experimental systems used in the current studies. b–d The influences of sh-Dnmt1 and Tet1 on the methylation levels of CpG sites near TSS (？1.5？~？+？2.0？kb) (b), methylation levels of all protein-coding genes (c), and iPSC generation (d) were summarized. e–j CpG sites (near TSS, ？1.5？~？+？2.0？kb) and genes with a larger demethylation than average were further selected. The overlapping targets of the two types of DNA demethylation were summarized in e–h. In addition, the correlations between demethylation induced by sh-Dnmt1 and Tet1 were listed in f and i. Consistently demethylated CpG sites near TSS (approximately 82,000, g) and genes (1680, j) were summarized by overlapping the results shown in e and h. k–m Dnmt1 and sh-Dnmt1 were over-expressed with Tet1. The overall methylation levels of the CpG sites near TSS (？1.5？~？+？2.0？kb) (k), overall methylation levels of all protein-coding genes (l), and iPSC generation (m) were summarize