Neutrophil extracellular traps induced by VP1 contribute to pulmonary edema during EV71 infection

a The effects of VP1 on pulmonary edema were determined by the wet-to-dry (W/D) lung weight ratio 4？h after VP1 intranasally administered. b Relative mRNA expression (log2) of inflammatory cytokines (TNF-α, IL-1β, and IL-6) in lungs was determined by qRT-PCR. c Representative images of H&E‐stained lung sections for the analysis of inflammatory cell infiltration and pulmonary edema; magnification, ×200; scale bar, 100？μm. The data are shown as the mean？±？SEM. n？=？3–5 per group. *p？<？0.05, **p？<？0.01, ***p？<？0.000