全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
-  2019 

Environmental stress impairs photoreceptor outer segment (POS) phagocytosis and degradation and induces autofluorescent material accumulation in hiPSC-RPE cells

DOI: 10.1038/s41420-019-0171-9

Keywords: Stem cells, Pluripotent stem cells

Full-Text   Cite this paper   Add to My Lib

Abstract:

a Representative electron microscopy images depicting the presence of RPE-characteristic microvilli structures and apically migrating melanosomes in all hiPSC-RPE lines, lines 1–3 (scale bar?=?1?μm). b Graphical representation of transepithelial resistance (TER) measurements showing TER?≥?150?Ω?cm2 (dotted line; the reported in vivo TER threshold40) in all hiPSC-RPE lines. Data are represented as mean?±?SEM. c Representative confocal images displaying the expected localization of tight junction marker, ZO-1, and apically expressed RPE protein, EZR, in all hiPSC-RPE lines (scale bar?=?20?μm). d Representative western blot images showing robust expression of several RPE signature proteins (BEST1, CRALBP, EZR, and RPE65) in all hiPSC-RPE lines. Of note, ACTN served as a loading control for western blot analyses. e Light microscopy images showing the presence of fluid domes, a characteristic of polarized epithelium, in hiPSC-RPE lines grown on nonporous tissue culture plastic support. Of note, the RPE morphology in focal plane 1 can be seen in the RPE monolayer outside the fluid dome, whereas the RPE cells within the fluid dome can be clearly seen in focal plane 2 (scale bar?=?250?μm

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133