%0 Journal Article
%T Environmental stress impairs photoreceptor outer segment (POS) phagocytosis and degradation and induces autofluorescent material accumulation in hiPSC-RPE cells
%A Ali Hashim
%A Celia Soto
%A Chad A. Galloway
%A Cynthia Tang
%A Lauren Winschel
%A Leslie A. MacDonald
%A Ruchira Singh
%A Sonal Dalvi
%J Archive of "Cell Death Discovery".
%D 2019
%R 10.1038/s41420-019-0171-9
%X a Representative electron microscopy images depicting the presence of RPE-characteristic microvilli structures and apically migrating melanosomes in all hiPSC-RPE lines, lines 1¨C3 (scale bar£¿=£¿1£¿¦Ìm). b Graphical representation of transepithelial resistance (TER) measurements showing TER£¿¡Ý£¿150£¿¦¸£¿cm2 (dotted line; the reported in vivo TER threshold40) in all hiPSC-RPE lines. Data are represented as mean£¿¡À£¿SEM. c Representative confocal images displaying the expected localization of tight junction marker, ZO-1, and apically expressed RPE protein, EZR, in all hiPSC-RPE lines (scale bar£¿=£¿20£¿¦Ìm). d Representative western blot images showing robust expression of several RPE signature proteins (BEST1, CRALBP, EZR, and RPE65) in all hiPSC-RPE lines. Of note, ACTN served as a loading control for western blot analyses. e Light microscopy images showing the presence of fluid domes, a characteristic of polarized epithelium, in hiPSC-RPE lines grown on nonporous tissue culture plastic support. Of note, the RPE morphology in focal plane 1 can be seen in the RPE monolayer outside the fluid dome, whereas the RPE cells within the fluid dome can be clearly seen in focal plane 2 (scale bar£¿=£¿250£¿¦Ìm
%K Stem cells
%K Pluripotent stem cells
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6522536/