M2 receptors activation modulates cell growth, migration and differentiation of rat Schwann-like adipose-derived stem cells

a Stem cell markers expression was assessed by RT-PCR. ASCs were used as positive control for stem cell markers expression. After growth factors exposure, stemness markers, CD90, CD44 and CD29 were significantly downregulated in dASCs (***p？<？0.001). Data are represented as mean ± SEM of three independent experiments. b Glial markers expression was evaluated by RT-PCR. ASCs were used as negative control, while nerve-derived SCs were used as positive control. After 18 days of differentiation, glial markers were upregulated in dASCs (i.e., Sox10, S100β, GFAP and P0). c Analysis in contrast phase microscopy showed that dASCs changed their morphology compared to ASCs, featuring the classical spindle-shaped morphology similar to SCs (scale bar: 200？μm). d Expression of S100β and GFAP in dASCs was analysed by immunocytochemistry (scale bar: 100？μm