%0 Journal Article
%T M2 receptors activation modulates cell growth, migration and differentiation of rat Schwann-like adipose-derived stem cells
%A Ada Maria Tata
%A Adam J. Reid
%A Alessandro Faroni
%A Roberta Piovesana
%A Valerio Magnaghi
%J Archive of "Cell Death Discovery".
%D 2019
%R 10.1038/s41420-019-0174-6
%X a Stem cell markers expression was assessed by RT-PCR. ASCs were used as positive control for stem cell markers expression. After growth factors exposure, stemness markers, CD90, CD44 and CD29 were significantly downregulated in dASCs (***p£¿<£¿0.001). Data are represented as mean ¡À SEM of three independent experiments. b Glial markers expression was evaluated by RT-PCR. ASCs were used as negative control, while nerve-derived SCs were used as positive control. After 18 days of differentiation, glial markers were upregulated in dASCs (i.e., Sox10, S100¦Â, GFAP and P0). c Analysis in contrast phase microscopy showed that dASCs changed their morphology compared to ASCs, featuring the classical spindle-shaped morphology similar to SCs (scale bar: 200£¿¦Ìm). d Expression of S100¦Â and GFAP in dASCs was analysed by immunocytochemistry (scale bar: 100£¿¦Ìm
%K Peripheral nervous system
%K Stem-cell research
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6499790/