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-  2019 

Kremen1-induced cell death is regulated by homo- and heterodimerization

DOI: 10.1038/s41420-019-0175-5

Keywords: Apoptosis, Cancer, Cell signalling, Cell death

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Abstract:

a Lysates from HEK293T cells transfected with Flag-Krm1 and HA-tagged full-length or truncation mutants of Krm1 lacking either the extracellular domain (ΔECD), the intracellular domain (ΔICD), or the transmembrane and intracellular domains (secECD) and subjected to anti-Flag immunoprecipitation followed by western blot using an anti-HA. An anti-panCadherin was used as control. Straight western blots on lysates are shown on the bottom panels. b Surface (green) and total (red) HA immunostaining of HEK293T cells transfected with HA-Krm1secECD together with either Green Fluorescent Protein (GFP, top lane) or full-length Krm1 (bottom lane). Scale bar: 10?μm. c Western blots and immunoprecipitation following extracellular proteins crosslinking with BS3 of HEK293T cells transfected with HA-Krm1 and Flag-Krm

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