%0 Journal Article
%T Kremen1-induced cell death is regulated by homo- and heterodimerization
%A Alessandra Pierani
%A Fr¨¦d¨¦ric Causeret
%A Iffat Sumia
%J Archive of "Cell Death Discovery".
%D 2019
%R 10.1038/s41420-019-0175-5
%X a Lysates from HEK293T cells transfected with Flag-Krm1 and HA-tagged full-length or truncation mutants of Krm1 lacking either the extracellular domain (¦¤ECD), the intracellular domain (¦¤ICD), or the transmembrane and intracellular domains (secECD) and subjected to anti-Flag immunoprecipitation followed by western blot using an anti-HA. An anti-panCadherin was used as control. Straight western blots on lysates are shown on the bottom panels. b Surface (green) and total (red) HA immunostaining of HEK293T cells transfected with HA-Krm1secECD together with either Green Fluorescent Protein (GFP, top lane) or full-length Krm1 (bottom lane). Scale bar: 10£¿¦Ìm. c Western blots and immunoprecipitation following extracellular proteins crosslinking with BS3 of HEK293T cells transfected with HA-Krm1 and Flag-Krm
%K Apoptosis
%K Cancer
%K Cell signalling
%K Cell death
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6494814/