Sirt6-induced autophagy restricted TREM-1-mediated pyroptosis in ox-LDL-treated endothelial cells: relevance to prognostication of patients with acute myocardial infarction

a Confocal detection of ox-LDL-induced pyroptotic cells formation. LDL was used as a negative control, and LPS as a positive control. Green: 488-labeled Casp1; red: TMR red-labeled pyroptotic nucleus; blue: DAPI-labeled natural nucleus; green and red double-labeled cells correspond to pyroptotic cells. N？=？3. Scale bar: 50？μm. b Flow cytometry detecting ox-LDL-induced pyroptotic cells percentage. N？=？3; *p？<？0.05 versus LDL group. c. Flow cytometry detection of ox-LDL-induced EMPs number. N？=？3; *p？<？0.05 versus LDL group. d RT-PCR detection of mRNA expression of ox-LDL-induced inflammatory factors. N？=？3; *p？<？0.05 versus LDL grou