Targeting JNK pathway promotes human hematopoietic stem cell expansion

a Experimental schematic for the evaluation of JNK inhibitors on HSC expansion. CD34+ cells were cultured with StemSpan SFEM II medium supplemented with SCF, TPO, FLT3L in the presence of JNK-related molecules for 7 days, then the total cell expansion and CD34+CD45RA- cell frequency was determined. b Frequency of CD34+CD45RA- cell subsets in 7-day cultures of CD34+ cells supplemented with two representative JNK inhibitors including JNK-IN-8 (2？μM) and SP600125 (5？μM) (n？=？3 experiments). c Chemical structure for JNK-IN-8 (hereafter called J8). d The increasing fold of CD34+CD45RA- subset frequency compared to DMSO after 10-day culture of CD34+ cells with different concentration of J8 (This data was drawn by R). See also to Supplementary Table S1. All data shown as mean values？±？SD. Statistical significance was assessed using unpaired t test, where ***p？<？0.001. See also Supplementary Fig. S