%0 Journal Article
%T Targeting JNK pathway promotes human hematopoietic stem cell expansion
%A Chengyan Wang
%A Hongkui Deng
%A Huangfan Xie
%A Jingliang Zhang
%A Lijun Shang
%A Ming Yin
%A Minhui Shi
%A Weifeng Lai
%A Weijie Guo
%A Wenhan Chen
%A Xiong Xiao
%A Yang Liu
%A Yifang Liu
%A Yu Li
%A Yuanjun Li
%J Archive of "Cell Discovery".
%D 2019
%R 10.1038/s41421-018-0072-8
%X a Experimental schematic for the evaluation of JNK inhibitors on HSC expansion. CD34+ cells were cultured with StemSpan SFEM II medium supplemented with SCF, TPO, FLT3L in the presence of JNK-related molecules for 7 days, then the total cell expansion and CD34+CD45RA- cell frequency was determined. b Frequency of CD34+CD45RA- cell subsets in 7-day cultures of CD34+ cells supplemented with two representative JNK inhibitors including JNK-IN-8 (2£¿¦ÌM) and SP600125 (5£¿¦ÌM) (n£¿=£¿3 experiments). c Chemical structure for JNK-IN-8 (hereafter called J8). d The increasing fold of CD34+CD45RA- subset frequency compared to DMSO after 10-day culture of CD34+ cells with different concentration of J8 (This data was drawn by R). See also to Supplementary Table S1. All data shown as mean values£¿¡À£¿SD. Statistical significance was assessed using unpaired t test, where ***p£¿<£¿0.001. See also Supplementary Fig. S
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6323118/