%0 Journal Article %T Targeting JNK pathway promotes human hematopoietic stem cell expansion %A Chengyan Wang %A Hongkui Deng %A Huangfan Xie %A Jingliang Zhang %A Lijun Shang %A Ming Yin %A Minhui Shi %A Weifeng Lai %A Weijie Guo %A Wenhan Chen %A Xiong Xiao %A Yang Liu %A Yifang Liu %A Yu Li %A Yuanjun Li %J Archive of "Cell Discovery". %D 2019 %R 10.1038/s41421-018-0072-8 %X a Experimental schematic for the evaluation of JNK inhibitors on HSC expansion. CD34+ cells were cultured with StemSpan SFEM II medium supplemented with SCF, TPO, FLT3L in the presence of JNK-related molecules for 7 days, then the total cell expansion and CD34+CD45RA- cell frequency was determined. b Frequency of CD34+CD45RA- cell subsets in 7-day cultures of CD34+ cells supplemented with two representative JNK inhibitors including JNK-IN-8 (2£¿¦ÌM) and SP600125 (5£¿¦ÌM) (n£¿=£¿3 experiments). c Chemical structure for JNK-IN-8 (hereafter called J8). d The increasing fold of CD34+CD45RA- subset frequency compared to DMSO after 10-day culture of CD34+ cells with different concentration of J8 (This data was drawn by R). See also to Supplementary Table S1. All data shown as mean values£¿¡À£¿SD. Statistical significance was assessed using unpaired t test, where ***p£¿<£¿0.001. See also Supplementary Fig. S %U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6323118/