SG formation relies on eIF4GI-G3BP interaction which is targeted by picornavirus stress antagonists

a–c Effects of eIF4GI KD on tSG formation. Examination of the protein level of eIF4GI in HeLa cells with KD of eIF4GI (a). Cells were treated with AS for 1？h, then fixed and stained with G3BP (red) and TIA-1 (green). IF (b) and quantitation (c) analysis of cells with tSGs (G3BP served as a marker). n？=？3, 300 cells/assay were counted, mean？±？SD; ***p？<？0.001. d–f Effects of eIF4GI KO on tSG formation. Examination of the protein level of eIF4GI in HeLa cells with KO of eIF4GI (HeLa-eIF4GI-KO cells) (d). Cells were treated with AS for 1？h and then fixed and stained with eIF4GI (purple), G3BP (red), and TIA-1 (green). IF (e) and quantitation (f) analysis of cells with tSGs (G3BP served as a marker). n？=？3, 300 cells/assay were counted, mean？±？SD; ***p？<？0.001. Scale bars, 10？μm. See also Supplementary Fig. S