%0 Journal Article
%T SG formation relies on eIF4GI-G3BP interaction which is targeted by picornavirus stress antagonists
%A Dong Guo
%A Mingzhou Chen
%A Qiang Zhang
%A Shanshan Fan
%A Xiaodan Yang
%A Yali Qin
%A Yi Zhong
%A Zhulong Hu
%J Archive of "Cell Discovery".
%D 2019
%R 10.1038/s41421-018-0068-4
%X a¨Cc Effects of eIF4GI KD on tSG formation. Examination of the protein level of eIF4GI in HeLa cells with KD of eIF4GI (a). Cells were treated with AS for 1£¿h, then fixed and stained with G3BP (red) and TIA-1 (green). IF (b) and quantitation (c) analysis of cells with tSGs (G3BP served as a marker). n£¿=£¿3, 300 cells/assay were counted, mean£¿¡À£¿SD; ***p£¿<£¿0.001. d¨Cf Effects of eIF4GI KO on tSG formation. Examination of the protein level of eIF4GI in HeLa cells with KO of eIF4GI (HeLa-eIF4GI-KO cells) (d). Cells were treated with AS for 1£¿h and then fixed and stained with eIF4GI (purple), G3BP (red), and TIA-1 (green). IF (e) and quantitation (f) analysis of cells with tSGs (G3BP served as a marker). n£¿=£¿3, 300 cells/assay were counted, mean£¿¡À£¿SD; ***p£¿<£¿0.001. Scale bars, 10£¿¦Ìm. See also Supplementary Fig. S
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312541/