Systemically administered peptain-1 inhibits retinal ganglion cell death in animal models: implications for neuroprotection in glaucoma

a αB-Crystallin (red) was detected in the nerve fiber layer (NFL) and in the outer plexiform layer (OPL) in the non-glaucomatous retinas. Only very weak immunostaining in the NFL was observed in the glaucomatous retinas. Representative images from the human retinas. The RGCs were labeled with a Brn3a antibody (green). The cell nuclei were stained with DAPI (blue). Scale bar？=？50？μm. b The number of RGCs in the retinas of the CRYAB KO mice was lower than that in the retinas of the age-matched WT mice. The bar graphs represent the means？±？SD of triplicate measurements. Scale bar？=？200？μm. ns not significant, *p？<？0.05 (unpaired t-test). GLC？=？ganglion cell laye