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-  2019 

24(S)-Hydroxycholesterol induces ER dysfunction-mediated unconventional cell death

DOI: 10.1038/s41420-019-0192-4

Keywords: Apoptosis, Sterols

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Abstract:

a–c SH-SY5Y cells were pretreated with 5?μM {"type":"entrez-nucleotide","attrs":{"text":"F12511","term_id":"708507"}}F12511 for 15?min or with 100?μM Nec-1 (a) for 1?h and then exposed to 50?μM 24S-OHC for 6?h. Cells were also treated with 3?μM thapsigargin (Thapsi) for 3?h. a Whole-cell lysates were subjected to immunoblotting with appropriate antibodies as indicated. b The unspliced (XBP1u) and spliced (XBP1s) XBP1 mRNAs were analyzed by RT-PCR. c Whole-cell lysates were immunoblotted with antibodies specific for XBP1s or β-actin. d Cells were pretreated with 20?μM MG132 for 30?min and then exposed to 50?μM 24S-OHC or 3?μM thapsigargin for 6?h. Whole-cell lysates were immunoblotted with antibodies specific for ATF6 or β-actin. Asterisks denote nonspecific bands. e, f Cells were treated as in panel a. g, h Cells were treated as in panel d. e, g Whole-cell lysates were subjected to immunoblotting with appropriate antibodies as indicated. f, h Band intensities were quantified by densitometric scanning, relative intensity is shown. Mean?±?SD n?=?3, **P?<?0.01, n.s. not significan

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