%0 Journal Article
%T 24(S)-Hydroxycholesterol induces ER dysfunction-mediated unconventional cell death
%A Araki Hirofumi
%A Diep-Khanh Ho Vo
%A Noriko Noguchi
%A Yasuomi Urano
%J Archive of "Cell Death Discovery".
%D 2019
%R 10.1038/s41420-019-0192-4
%X a¨Cc SH-SY5Y cells were pretreated with 5£¿¦ÌM {"type":"entrez-nucleotide","attrs":{"text":"F12511","term_id":"708507"}}F12511 for 15£¿min or with 100£¿¦ÌM Nec-1 (a) for 1£¿h and then exposed to 50£¿¦ÌM 24S-OHC for 6£¿h. Cells were also treated with 3£¿¦ÌM thapsigargin (Thapsi) for 3£¿h. a Whole-cell lysates were subjected to immunoblotting with appropriate antibodies as indicated. b The unspliced (XBP1u) and spliced (XBP1s) XBP1 mRNAs were analyzed by RT-PCR. c Whole-cell lysates were immunoblotted with antibodies specific for XBP1s or ¦Â-actin. d Cells were pretreated with 20£¿¦ÌM MG132 for 30£¿min and then exposed to 50£¿¦ÌM 24S-OHC or 3£¿¦ÌM thapsigargin for 6£¿h. Whole-cell lysates were immunoblotted with antibodies specific for ATF6 or ¦Â-actin. Asterisks denote nonspecific bands. e, f Cells were treated as in panel a. g, h Cells were treated as in panel d. e, g Whole-cell lysates were subjected to immunoblotting with appropriate antibodies as indicated. f, h Band intensities were quantified by densitometric scanning, relative intensity is shown. Mean£¿¡À£¿SD n£¿=£¿3, **P£¿<£¿0.01, n.s. not significan
%K Apoptosis
%K Sterols
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6611791/