Genetic variation may confound analysis of CRISPR-Cas9 off-target mutations

a Venn diagrams displaying the number of SNVs (left) or indels (right) identified in the CRISPR-edited animal and control mice. B6-1, B6-2, and B6-3: C57BL/6J control mice. CRISPR-edited: Zkscan1+/？ mouse. b Heat maps showing the number of SNV (green) and indel (purple) sites at which the experimental mice have different genotypes compared with each other. c Number of SNV or indel sites located at 3-bp upstream from an NGG sequence (on both strands