Nicotinamide mononucleotide promotes osteogenesis and reduces adipogenesis by regulating mesenchymal stromal cells via the SIRT1 pathway in aged bone marrow

a Scheme of NMN administration and the CFU-F assay. b Representative images acquired from live cell imaging system showing CFU-F colonies formed by MSCs. c The number of CFU-F contained in MSCs. d Scheme of NMN administration and flow cytometric analysis. e, f The PDGFRα+ MSCs (e), and LepR+ MSCs (f) from enzymatically dissociated femur bone marrow cells are shown. g The frequencies of LepR+ MSCs, PDGFRα+ MSCs, and PDGFRα+ LepR+ MSCs from enzymatically dissociated bone marrow cells. h The cellularity of the enzymatically dissociated bone marrow cells (n？=？6 mice per group from three independent experiments). *p？<？0.05, **p？<？0.01 error bars, s.d