SVIP alleviates CCl4-induced liver fibrosis via activating autophagy and protecting hepatocytes

a Western blots (WB) and b PCR showing the expression of Beclin1, p62, LC3B, Atg5 in HepG2 cells transfected with SVIP expressing or control plasmid. c and d Western blots showing the expression of TFEB, Rab7, and conversion of LC3BII to LC3BI (c) in cells transfected with SVIP expressing or/and control plasmid in different dose (d). HepG2 cells were transfected with 2？μg total DNA, containing SVIP-expressing plasmid or vector control or 1？μg SVIP-expressing plasmid？+？1？μg vector control. Baf-A1, (100？ng/ml) Bafilomycin A1. e Western blots and f PCR showing the expression of Beclin1, p62, LC3B, Atg5 in silence SVIP HepG2 cells. 24 and 72？h after the plasmid and siRNA transfection, cells were subjected to WB and reverse-transcriptional PCR, respectively. Bar graph indicates the bands’ intensity of mRNA calibrated with β-actin RNA level. LC3-II/LC3-I ratio represents the autophagy flux. Data are presented as mean？±？standard division (SD) in three independent experiments. *p？<？0.05; **p？<？0.0