%0 Journal Article
%T SVIP alleviates CCl4-induced liver fibrosis via activating autophagy and protecting hepatocytes
%A Caihua Zhang
%A Chuandong Cheng
%A Cong Li
%A Dan Jia
%A David Yuke Liang
%A Dongmei Wang
%A Lianying Guo
%A Pin Liang
%A Pin Wang
%A Yang Wang
%A Yao Huang
%A Yuan Yuan Wang
%A Yujie Jia
%J Archive of "Cell Death & Disease".
%D 2019
%R 10.1038/s41419-019-1311-0
%X a Western blots (WB) and b PCR showing the expression of Beclin1, p62, LC3B, Atg5 in HepG2 cells transfected with SVIP expressing or control plasmid. c and d Western blots showing the expression of TFEB, Rab7, and conversion of LC3BII to LC3BI (c) in cells transfected with SVIP expressing or/and control plasmid in different dose (d). HepG2 cells were transfected with 2£¿¦Ìg total DNA, containing SVIP-expressing plasmid or vector control or 1£¿¦Ìg SVIP-expressing plasmid£¿+£¿1£¿¦Ìg vector control. Baf-A1, (100£¿ng/ml) Bafilomycin A1. e Western blots and f PCR showing the expression of Beclin1, p62, LC3B, Atg5 in silence SVIP HepG2 cells. 24 and 72£¿h after the plasmid and siRNA transfection, cells were subjected to WB and reverse-transcriptional PCR, respectively. Bar graph indicates the bands¡¯ intensity of mRNA calibrated with ¦Â-actin RNA level. LC3-II/LC3-I ratio represents the autophagy flux. Data are presented as mean£¿¡À£¿standard division (SD) in three independent experiments. *p£¿<£¿0.05; **p£¿<£¿0.0
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6347612/