Regrowth-delay body as a bacterial subcellular structure marking multidrug-tolerant persisters

a Immunoblotting results for detecting endogenous FtsZ, EF-Tu, or GroEL in the total cell lysate (total), supernatant (sup.) and pellet (pel.) of the log-phase or late stationary-phase wild-type E. coli cells, probed with the indicated antibodies. b Fluorescence and bright field microscopic images of the log-phase (top) and late stationary-phase (bottom) E. coli cells in which FtsZ-mNeonGreen was heterologously expressed. Scale bars, 1？μm. c Fluorescence microscopic images of the log-phase and late stationary-phase ftsZ-mNeonGreen-dnaK-mCherry or ftsZ-mNeonGreen-clpB-mCherry cells. Scale bars, 1？μm. d Fluorescence microscopic images of the late stationary-phase ftsZ-mNeonGreen cells in which the FtsZ inhibitor protein CbtA was expressed (left panel) Scale bars, 1？μm; the corresponding immunoblotting results for detecting FtsZ in the indicated cell lysate fractions, probed with anti-FtsZ antibodies (right panel