%0 Journal Article
%T Regrowth-delay body as a bacterial subcellular structure marking multidrug-tolerant persisters
%A Huijia Yin
%A Jiayu Yu
%A Yang Liu
%A Zengyi Chang
%J Archive of "Cell Discovery".
%D 2019
%R 10.1038/s41421-019-0080-3
%X a Immunoblotting results for detecting endogenous FtsZ, EF-Tu, or GroEL in the total cell lysate (total), supernatant (sup.) and pellet (pel.) of the log-phase or late stationary-phase wild-type E. coli cells, probed with the indicated antibodies. b Fluorescence and bright field microscopic images of the log-phase (top) and late stationary-phase (bottom) E. coli cells in which FtsZ-mNeonGreen was heterologously expressed. Scale bars, 1£¿¦Ìm. c Fluorescence microscopic images of the log-phase and late stationary-phase ftsZ-mNeonGreen-dnaK-mCherry or ftsZ-mNeonGreen-clpB-mCherry cells. Scale bars, 1£¿¦Ìm. d Fluorescence microscopic images of the late stationary-phase ftsZ-mNeonGreen cells in which the FtsZ inhibitor protein CbtA was expressed (left panel) Scale bars, 1£¿¦Ìm; the corresponding immunoblotting results for detecting FtsZ in the indicated cell lysate fractions, probed with anti-FtsZ antibodies (right panel
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6341109/