Targeting Akt by SC66 triggers GSK-3β mediated apoptosis in colon cancer therapy

SC66 inhibited colon cancer cell proliferation in vitro. a Cell viability of HCT-116 was detected using cell counting kit-8 after 0, 0.5, 1, 2 or 4 μg/ml SC66 treatment at indicated time. Data represent the mean？±？SEM of four independent experiments. b Cell viability was analyzed in various colon cancer cells after 2 μg/ml SC66 treatment for 24 h. Data represent the mean？±？SEM of four independent experiments. c Western blotting analysis of P-Akt and Akt expression in HCT-116 WT treated with 2 μg/ml SC66 treatment for 24 h. d Colony formation was analyzed by crystal violet staining in HCT-116 WT, HCT-116 p53？/？ and DLD1 cells following 2 μg/ml SC66 treatment for 24 h. Similar results were obtained from three independent experiment