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-  2019 

Monocarboxylate transporter 1 and monocarboxylate transporter 4 in cancer-endothelial co-culturing microenvironments promote proliferation, migration, and invasion of renal cancer cells

DOI: 10.1186/s12935-019-0889-8

Keywords: Monocarboxylate transporter, Glycolytic metabolism, Renal cell carcinoma, Cancer-endothelial microenvironment, Cancer invasion, Cancer metastasis

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Abstract:

The viability of 786-O cells and HUVECs in the co-culture mode and the control single-culture mode. a, b In the transwell culturing, 1?×?104 cells were seeded in the upper chamber and 4?×?104 cells were seeded in the lower chamber. The viability of (a) 786-O cells and (b) HUVECs was measured by a CCK-8 assay at 0, 24, 48, 72, and 96 h after culturing. For the control, the cells were seeded in both the upper and lower chambers; for the HUVEC coculture, 786-O cells were added to the upper chamber while HUVECs were added to the lower chamber; for the 786-O coculture, HUVECs were added to the upper chamber while 786-O cells were added to the lower chamber; and, for the control?+?7ACC1 or coculture?+?7ACC1, 10 μM 7ACC1 was added to the culturing conditions. *P?<?0.001, compared with the contro

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