New Substituted Cyanoindoline Derivatives as MAP3K14 Kinase Inhibitors for the Treatment of Cancer and Autoimmune Disorders

The NIK/MAP3K14 autophosphorylation activity was measured using the Alpha Screen (αscreen) format (PerkinElmer). The compounds were tested for inhibition of the autophosphorylation of recombinant human NF-kappaB-inducing kinase (NIK/MAP3K14) activity. Assays were carried out in 384-well Alphaplates (PerkinElmer)