脂肪酸结合蛋白5和细胞维甲酸结合蛋白2在脑胶质瘤中的表达
DOI: 10.3971/j.issn.1000-8578.2015.03.007
Keywords: 105 Cases of Glioma,ERK2 Expression in Human Glioma Tissues and Its Clinical Significance,Effects of Rendezvous Chemotherapy Combined with Three-dimensional Conformal#br# Radiotherapy in Treatment for Post-operative Malignant Glioma,Expression and Signifi cance of Smad-1, Smad-2 and Smad-4 in Human Glioma,Study of Time-Domain of Blood-brain Barrier Opened and Tight Junction Changed in Glioma Models after Whole-brain Irradiated with Single Dose,Expression of EGFL7 in Human Gliomas and Its Correlation with Glioma Grade,Postoperative Intensity Modulated Radiation Therapy for 30 Cases with Malignant Gliomas,Construction of LRIG1 Specific RNAi Expressing Vector and Screening of Stably Transfected Cell Clone,Primary Culture and Drug Sensitivity of Human Glioma Cells,Inhibitive Effect of Artemether on Glioma Growth in SD Rat Orthotopic Glioma Model,Inhibitive Effect of Artemether on Brain Glioma Angiogenesis in SD Rat
Abstract:
摘要 目的 探讨脂肪酸结合蛋白5（fatty acid-binding protein 5，FABP5）与细胞维甲酸结合蛋白2（cellular retinoic acid-binding protein 2，CRABP2）在脑胶质瘤中的表达及与脑胶质瘤病理级别、维甲酸治疗抵抗的关系。方法 应用免疫组织化学染色法检测125例脑星型细胞瘤组织中FABP5及CRABP2蛋白表达。全反式维甲酸作用前后，以Brdu-ELISA法检测胶质瘤细胞增殖，并以实时荧光定量PCR检测胶质瘤细胞株中FABP5及CRABP2在mRNA水平的表达。结果 FABP5阳性细胞比例在WHO Ⅱ级星型细胞瘤中占（16±9）%，Ⅲ级中占（33±22）%，Ⅳ级中占（50±29）%，FABP5蛋白表达与胶质瘤病理级别呈显著正相关（P<0.05）。CRABP2阳性细胞比例在Ⅱ级星型细胞瘤中占（46±12）%，WHO Ⅲ级中占（30±15）%，Ⅳ级中占（10±9）%，CRABP2蛋白表达与胶质瘤病理级别呈显著负相关（P<0.05）。全反式维甲酸促进了胶质瘤细胞株的增殖，全反式维甲酸作用后胶质瘤细胞FABP5 mRNA表达显著上调，而CRABP2显著下调。 结论 FABP5及CRABP2的异常表达与胶质瘤恶性程度相关，并可能介导了胶质瘤细胞对维甲酸的分化抵抗效应
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