大肠癌细胞FoxQ1与EGFR基因表达的相关性
DOI: 10.3971/j.issn.1000-8578.2016.01.005
Keywords: FoxQ1基因,表皮生长因子受体,RNA干扰,EGFR酪氨酸激酶抑制,Resveratrol Inhibits Non-small Cell Lung Cancer via Directly Targeting EGFR and c-Met Signaling Pathways,Effect of Down-regulating KLF8 Expression by siRNA on EMT in Nasopharyngeal Carcinoma Cells,Effect of Gene Silencing of RNF2 on Radiosensitivity of Esophageal Carcinoma Cells,Effect of RNA Interference Decreasing MSI1 Expression on Chemosensitivity of Human Glioma Cells,Predictive Factors for Customizing Chemotherapy on Advanced Lung Adenocarcinoma,MDM2 siRNA Inhibits Angiogenesis by Reducing Vascular Endothelial Growth Factor Production in Breast Cancer Cells,Review of Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitors in Adjuvant Treatment of Non-small Cell Lung Cancer,Correlation Between Epithelial-mesenchymal Transition and Sensitivity to EGFR Targeted Monoclonal Antibody in Triple Negative Breast Cancer Cells,In vitro Investigation on Effect of Lentiviral Vector-mediated RNA Interference Inhibiting VASH1 Expression on Proliferation and Apoptosis of Human Glioma Cells U-87MG,Effect of exosome Extracted from Fibroblast Cell Line 3T3 on Proliferation of Mouse Breast Cancer Cells,Clinical Observation of Trastuzumab Across Multiple Lines plus Different Chemotherapy Regimens on HER2 Positive Advanced Breast Cancer Patients,Construction and Screening of shRNA Expression Plasmids Targeting at cortactin Gene,Down-regulating UHRF1 Expression could Inhibit Progression of Hepatocellular Carcinoma,Review on Relationship Between HER2 and Fatty Acid Synthase in Tumor Cells,Relationship of Clinical Characteristics and EGFR, K-RAS Mutation in Lung Adenocarcinoma Patients with Predominant Ground-glass Opacity
Abstract:
摘要 目的 探讨大肠癌细胞中FoxQ1与EGFR基因间的相关性，为研究大肠癌中FoxQ1基因在EGFR通路中的作用机制奠定基础。 方法 应用荧光定量PCR以293-T细胞中FoxQ1及EGFR基因相对表达量为1作为参照，检测大肠癌细胞系DLD1、HT29、LOVO、HCT116中FoxQ1及EGFR基因mRNA相对表达量；荧光定量检测经shRNA-FoxQ1慢病毒干扰后的DLD1细胞（命名为DLD1-shRNAFoxQ1）中EGFR的相对表达量改变；DLD1-shRNA-FoxQ1经EGFR酪氨酸激酶抑制剂Erlotinib HCl和siRNA-EGFR处理后，荧光定量PCR分别检测FoxQ1和EGFR基因mRNA相对表达量。结果 （1）FoxQ1在DLD1、HT29、LOVO、HCT116细胞系中的相对表达量分别为83.09、59.58、0.06、0.03，EGFR的相对表达量分别为4.95、3.67、2.08、1.36；（2）经shRNA-FoxQ1干扰的DLD1 细胞EGFR表达量随FoxQ1表达量的降低而增高；（3）细胞DLD1-shRNA-FoxQ1、DLD1-shRNA-Control分别经 siRNA-EGFR处理抑制EGFR的表达后，FoxQ1表达量随EGFR表达量的降低而增高，经Erlotinib HCl阻断EGFR酪氨酸激酶后，FoxQ1表达量增高。结论 大肠癌细胞系中FoxQ1与EGFR基因的表达趋势基本一致；同时两者间可能相互存在负反馈调节机制，从而维持大肠癌细胞中FoxQ1与EGFR高表达的状态
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