%0 Journal Article
%T 大肠癌细胞FoxQ1与EGFR基因表达的相关性
%A 唐慧
%A 白璇
%A 郭强
%J 肿瘤防治研究
%D 2016
%R 10.3971/j.issn.1000-8578.2016.01.005
%X 摘要 目的 探讨大肠癌细胞中FoxQ1与EGFR基因间的相关性，为研究大肠癌中FoxQ1基因在EGFR通路中的作用机制奠定基础。 方法 应用荧光定量PCR以293-T细胞中FoxQ1及EGFR基因相对表达量为1作为参照，检测大肠癌细胞系DLD1、HT29、LOVO、HCT116中FoxQ1及EGFR基因mRNA相对表达量；荧光定量检测经shRNA-FoxQ1慢病毒干扰后的DLD1细胞（命名为DLD1-shRNAFoxQ1）中EGFR的相对表达量改变；DLD1-shRNA-FoxQ1经EGFR酪氨酸激酶抑制剂Erlotinib HCl和siRNA-EGFR处理后，荧光定量PCR分别检测FoxQ1和EGFR基因mRNA相对表达量。结果 （1）FoxQ1在DLD1、HT29、LOVO、HCT116细胞系中的相对表达量分别为83.09、59.58、0.06、0.03，EGFR的相对表达量分别为4.95、3.67、2.08、1.36；（2）经shRNA-FoxQ1干扰的DLD1 细胞EGFR表达量随FoxQ1表达量的降低而增高；（3）细胞DLD1-shRNA-FoxQ1、DLD1-shRNA-Control分别经 siRNA-EGFR处理抑制EGFR的表达后，FoxQ1表达量随EGFR表达量的降低而增高，经Erlotinib HCl阻断EGFR酪氨酸激酶后，FoxQ1表达量增高。结论 大肠癌细胞系中FoxQ1与EGFR基因的表达趋势基本一致；同时两者间可能相互存在负反馈调节机制，从而维持大肠癌细胞中FoxQ1与EGFR高表达的状态
%K FoxQ1基因
%K 表皮生长因子受体
%K RNA干扰
%K EGFR酪氨酸激酶抑制
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%K Review of Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitors in Adjuvant Treatment of Non-small Cell Lung Cancer
%K Correlation Between Epithelial-mesenchymal Transition and Sensitivity to EGFR Targeted Monoclonal Antibody in Triple Negative Breast Cancer Cells
%K In vitro Investigation on Effect of Lentiviral Vector-mediated RNA Interference Inhibiting VASH1 Expression on Proliferation and Apoptosis of Human Glioma Cells U-87MG
%K Effect of exosome Extracted from Fibroblast Cell Line 3T3 on Proliferation of Mouse Breast Cancer Cells
%K Clinical Observation of Trastuzumab Across Multiple Lines plus Different Chemotherapy Regimens on HER2 Positive Advanced Breast Cancer Patients
%K Construction and Screening of shRNA Expression Plasmids Targeting at cortactin Gene
%K Down-regulating UHRF1 Expression could Inhibit Progression of Hepatocellular Carcinoma
%K Review on Relationship Between HER2 and Fatty Acid Synthase in Tumor Cells
%K Relationship of Clinical Characteristics and EGFR
%K K-RAS Mutation in Lung Adenocarcinoma Patients with Predominant Ground-glass Opacity
%U http://www.zlfzyj.com/CN/abstract/abstract8675.shtml