Gli抑制剂GANT61对肺癌细胞生长的抑制作用
DOI: 10.3971/j.issn.1000-8578.2016.02.004
Keywords: A Meta-analysis,Effect of Rap2a on Invasion and Metastasis of Lung Cancer Cells and Its Related Mechanism,Effect of GANT61 on Apoptosis of Esophageal Squamous Carcinoma Cells and Its Mechanism,NSCLC的疫苗治疗研究进展,Immune Killing Effect of Silencing TIPE2 Expression on T Lymphocytes Against Lung Adenocarcinoma Cells LA795,Clinical Investigation on Qingfei Mixture Combined with Chemotherapy on Middle and Advanced Non-small Cell Lung Cancer,Effect of Vascular Endothelial Cadherin on Invasion and Metastasis of Non-small Cell Lung Cancer Cells and Related Mechanism,Advances of FGF/FGFR Signaling Pathway in Targeted Therapy for Squamous Cell Lung Cancer,Sulforaphane Suppressed Proliferation of Lung Cancer Stem Cells in vitro,Experiment of Different Administration Sequences of Apatinib and Paclitaxel on Lung Cancer,Cryptotanshinone Promotes Apoptosis of A549 Cells via Inhibiting Cap-dependent mRNA Translation
Abstract:
摘要 目的 研究GANT61对人肺癌细胞H1703和A549生长抑制作用，并探讨其作用机制。方 法 培养H1703和A549细胞，加入不同浓度GANT61 72 h后MTS法检测药物对细胞生长的抑制率；Western blot法观察GANT61作用于H1703和A549细胞后对Gli1和Gli2蛋白表达的影响。Transwell侵袭实验观察GANT61作用于H1703和A549细胞后对侵袭能力的影响。结果 MTS显示GANT61作用于H1703和A549的IC50值分别是8.6 μmol/L和8.2 μmol/L，GANT61对H1703和A549细胞有明显的生长抑制作用并且呈剂量依赖性。Western blot显示GANT61可显著下调H1703和A549细胞Gli1和Gli2蛋白的表达。Transwell侵袭实验显示GANT61处理组H1703和A549细胞的侵袭能力均明显下降。结论 GANT61可以明显抑制H1703和A549的细胞活力和侵袭能力，这表明Hedgehog通路的Gli1和Gli2在肺癌的发生和发展中起着重要作用，Gli有望成为新的抗肿瘤靶点
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