%0 Journal Article
%T Gli抑制剂GANT61对肺癌细胞生长的抑制作用
%A 王林
%A 王雷
%A 米源
%J 肿瘤防治研究
%D 2016
%R 10.3971/j.issn.1000-8578.2016.02.004
%X 摘要 目的 研究GANT61对人肺癌细胞H1703和A549生长抑制作用，并探讨其作用机制。方 法 培养H1703和A549细胞，加入不同浓度GANT61 72 h后MTS法检测药物对细胞生长的抑制率；Western blot法观察GANT61作用于H1703和A549细胞后对Gli1和Gli2蛋白表达的影响。Transwell侵袭实验观察GANT61作用于H1703和A549细胞后对侵袭能力的影响。结果 MTS显示GANT61作用于H1703和A549的IC50值分别是8.6 μmol/L和8.2 μmol/L，GANT61对H1703和A549细胞有明显的生长抑制作用并且呈剂量依赖性。Western blot显示GANT61可显著下调H1703和A549细胞Gli1和Gli2蛋白的表达。Transwell侵袭实验显示GANT61处理组H1703和A549细胞的侵袭能力均明显下降。结论 GANT61可以明显抑制H1703和A549的细胞活力和侵袭能力，这表明Hedgehog通路的Gli1和Gli2在肺癌的发生和发展中起着重要作用，Gli有望成为新的抗肿瘤靶点
%K A Meta-analysis
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%K Effect of GANT61 on Apoptosis of Esophageal Squamous Carcinoma Cells and Its Mechanism
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%K Clinical Investigation on Qingfei Mixture Combined with Chemotherapy on Middle and Advanced Non-small Cell Lung Cancer
%K Effect of Vascular Endothelial Cadherin on Invasion and Metastasis of Non-small Cell Lung Cancer Cells and Related Mechanism
%K Advances of FGF/FGFR Signaling Pathway in Targeted Therapy for Squamous Cell Lung Cancer
%K Sulforaphane Suppressed Proliferation of Lung Cancer Stem Cells in vitro
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%K Cryptotanshinone Promotes Apoptosis of A549 Cells via Inhibiting Cap-dependent mRNA Translation
%U http://www.zlfzyj.com/CN/abstract/abstract8694.shtml