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- 2016
香加皮杠柳苷通过溶酶体途径诱导胃癌MGC-803细胞凋亡DOI: 10.3971/j.issn.1000-8578.2016.05.004 Keywords: 2 Matched Case-control Investigation on Risk Factors of Gastric Cancer in Xianyou County,Effects of FEN1 Overexpression on Biological Behaviors of Hepatocellular Carcinoma Cells and Prognosis of Patients,Effect of GANT61 on Apoptosis of Esophageal Squamous Carcinoma Cells and Its Mechanism,Progress of Relationships of Calprotectin with Tumours,Expression of CLIC4 in Gastric Carcinoma Tissues and Its Relevance to Microsatellite Instability,Experimental Investigation on Apoptosis of Cervical Cancer Cells Promoted by NF-κB Decoy ODN,Cryptotanshinone Promotes Apoptosis of A549 Cells via Inhibiting Cap-dependent mRNA Translation,Gastric Cancer Mortality Trend and Difference Decomposition Analysis in Kunshan, Jiangsu Province, 1981-2014 Abstract: 摘要 目的 探讨香加皮杠柳苷（periplocin extracted from cortex periplocae, CPP）是否通过溶酶体途径诱导胃癌细胞凋亡。方法 200、100、50 ng/ml香加皮杠柳苷作用于MGC-803细胞24和48 h后，MTS 法检测细胞的增殖情况，用TUNEL法检测细胞凋亡，Western blot检测不同浓度香加皮杠柳苷作用于MGC-803细胞24 h后Caspase-3和Caspase-9的表达情况，吖啶橙染色检测溶酶体释放情况，免疫荧光法检测组织蛋白酶B（Cathepsin B）的表达变化情况。结果 与空白对照组相比，50、100、200 ng/ml香加皮杠柳苷作用于MGC-803细胞24、48 h后，均具有增殖抑制作用，24 h增殖率分别为 [(66.43±1.67)%、(55.39±3.63)%、(37.06±0.57)% vs. (100.14±8.49)%，P均<0.05]，48 h增殖率分别为[(38.16±3.26)%、(32.00±1.83)%、(24.75±1.57)% vs. (100.33±8.98)%，P均<0.05]。与空白对照组相比，50、100、200 ng/ml香加皮杠柳苷作用于MGC-803细胞24 h后，细胞凋亡率显著增加，凋亡率为[(28.56±6.96)%、(33.70±4.60)%、(60.42±4.48)% vs. (2.23±1.03)%，P均<0.05]，Caspase-3的活性裂解片段和Caspase-9表达量升高，溶酶体膜的完整性被破坏，Cathepsin B由溶酶体释放到细胞质中。结论 香加皮杠柳苷可通过溶酶体途径诱导胃癌细胞凋亡
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