%0 Journal Article
%T 香加皮杠柳苷通过溶酶体途径诱导胃癌MGC-803细胞凋亡
%A 单保恩
%A 孙士萍
%A 崔雯萱
%A 张超
%A 彭克楠
%A 戴素丽
%A 李磊
%A 赵连梅
%J 肿瘤防治研究
%D 2016
%R 10.3971/j.issn.1000-8578.2016.05.004
%X 摘要 目的 探讨香加皮杠柳苷（periplocin extracted from cortex periplocae, CPP）是否通过溶酶体途径诱导胃癌细胞凋亡。方法 200、100、50 ng/ml香加皮杠柳苷作用于MGC-803细胞24和48 h后，MTS 法检测细胞的增殖情况，用TUNEL法检测细胞凋亡，Western blot检测不同浓度香加皮杠柳苷作用于MGC-803细胞24 h后Caspase-3和Caspase-9的表达情况，吖啶橙染色检测溶酶体释放情况，免疫荧光法检测组织蛋白酶B（Cathepsin B）的表达变化情况。结果 与空白对照组相比，50、100、200 ng/ml香加皮杠柳苷作用于MGC-803细胞24、48 h后，均具有增殖抑制作用，24 h增殖率分别为 [(66.43±1.67)%、(55.39±3.63)%、(37.06±0.57)% vs. (100.14±8.49)%，P均<0.05]，48 h增殖率分别为[(38.16±3.26)%、(32.00±1.83)%、(24.75±1.57)% vs. (100.33±8.98)%，P均<0.05]。与空白对照组相比，50、100、200 ng/ml香加皮杠柳苷作用于MGC-803细胞24 h后，细胞凋亡率显著增加，凋亡率为[(28.56±6.96)%、(33.70±4.60)%、(60.42±4.48)% vs. (2.23±1.03)%，P均<0.05]，Caspase-3的活性裂解片段和Caspase-9表达量升高，溶酶体膜的完整性被破坏，Cathepsin B由溶酶体释放到细胞质中。结论 香加皮杠柳苷可通过溶酶体途径诱导胃癌细胞凋亡
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%K Cryptotanshinone Promotes Apoptosis of A549 Cells via Inhibiting Cap-dependent mRNA Translation
%K Gastric Cancer Mortality Trend and Difference Decomposition Analysis in Kunshan
%K Jiangsu Province
%K 1981-2014
%U http://www.zlfzyj.com/CN/abstract/abstract8742.shtml