乳腺癌淋巴结微转移患者克隆性T细胞TCRα链CDR3谱型分析
DOI: 10.3971/j.issn.1000-8578.2015.11.011
Keywords: 28 Cases Report,MDM2 siRNA Inhibits Angiogenesis by Reducing Vascular Endothelial Growth Factor Production in Breast Cancer Cells,Difference of Self-renewal and Tumorigenicity among Different Breast Cancer Stem Cells Subpopulations,Relationship of ALDH1+/CD133+ Stem Cell-like Cells with Angiogenesis in Breast Invasive Ductal Carcinoma Tissues,Association between Urinary Molybdenum and Risk of Breast Cancer,Effects of Jinlong Capsules on Immune Functions of Breast Carcinoma Patients undergoing Postoperative Chemotherapy,Molecular Mechanisms of Docetaxel Resistance in Breast Cancer,Combined Expressions of Epithelial-Mesenchymal Transition Related Proteins in Triple-#br# Negative Breast Cancer and Its Prognostic Signifi cance,Acoustic Radiation Force Impulse for Differential Diagnosis of Benign or Malignant#br# Breast Tumors,Analysis on Related Factors of Bone Mineral Density Reduction in Postmenopausal#br# Women with Early Breast Cancer One Year after Surgery,Multivariate Analysis of Non-sentinel Lymph Node Status in Breast Cancer Patients with Positive Sentinel Lymph Node
Abstract:
摘要 目的 分析乳腺癌微转移淋巴结T细胞克隆性增生及TCR α链谱型偏移情况，了解抗肿瘤T细胞克隆的分子特征。方法 利用RT-PCR扩增10例乳腺癌微转移淋巴结T细胞32个TCR可变区（AV）亚家族的基因，基因扫描检测T细胞克隆性及TCR AV亚家族取用情况，对单克隆家族行PCR扩增TCR α链全长序列，构建重组质粒后测序，分析互补决定区（CDR3）序列。结果 乳腺癌淋巴结微转移患者T细胞呈单、寡克隆、寡克隆趋势和多克隆增生，不同患者表达1～4个TCR AV亚家族。克隆性T细胞的CDR3氨基酸序列不同，但是病例4和病例8含有相同的CDR3氨基酸基序：AM和DDKII。结论 乳腺癌TCR AV基因表达具有多样性特点，TCR AV家族的取用可能与乳腺癌肿瘤抗原的多样性和不同个体的免疫应答反应有关。相同CDR3氨基酸基序的发现可能对乳腺癌的T细胞介导免疫治疗提供帮助
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