%0 Journal Article
%T 乳腺癌淋巴结微转移患者克隆性T细胞TCRα链CDR3谱型分析
%A 于庆凯
%A 刘明阁
%A 吕晓东
%A 宋魏
%A 张建波
%A 房百俊
%A 胡骏
%J 肿瘤防治研究
%D 2015
%R 10.3971/j.issn.1000-8578.2015.11.011
%X 摘要 目的 分析乳腺癌微转移淋巴结T细胞克隆性增生及TCR α链谱型偏移情况，了解抗肿瘤T细胞克隆的分子特征。方法 利用RT-PCR扩增10例乳腺癌微转移淋巴结T细胞32个TCR可变区（AV）亚家族的基因，基因扫描检测T细胞克隆性及TCR AV亚家族取用情况，对单克隆家族行PCR扩增TCR α链全长序列，构建重组质粒后测序，分析互补决定区（CDR3）序列。结果 乳腺癌淋巴结微转移患者T细胞呈单、寡克隆、寡克隆趋势和多克隆增生，不同患者表达1～4个TCR AV亚家族。克隆性T细胞的CDR3氨基酸序列不同，但是病例4和病例8含有相同的CDR3氨基酸基序：AM和DDKII。结论 乳腺癌TCR AV基因表达具有多样性特点，TCR AV家族的取用可能与乳腺癌肿瘤抗原的多样性和不同个体的免疫应答反应有关。相同CDR3氨基酸基序的发现可能对乳腺癌的T细胞介导免疫治疗提供帮助
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%U http://www.zlfzyj.com/CN/abstract/abstract8634.shtml