重组质粒pCMV AIM-1 antisense对卵巢癌裸鼠移植瘤的抑瘤效应研究
DOI: 10.3971/j.issn.1000-8578.2015.11.006
Keywords: 重组质粒,AIM-1,移植瘤,裸鼠,Effect of Cisplatin Combined with Buzhong Yiqi Decoction on survivin Expression in Transplantation Tumor of Nude Mice with A549/DDP Cells,Effects of Different Doses of Bevacizumab on Irinotecan Distribution in Nude Mice Bearing Human Colon Cancer Cell Xenografts,Reversion Impact of Peptide Extract from Scorpion Venom on Multidrug-resistance of Leukemia Stem Cell Line K562/A02 in vivo,Inhibitory Effect of Silencing PCAF Gene on Growth and Angiogenesis of Human Adrenocortical Carcinoma Xenograft in Nude Mice,Effect of Silencing TLR4 Gene by RNA Interference Carried by Lentiviral Vector on Subcutaneous Tumor of Human Hepatitis B Virus-associated Hepatocellular Carcinoma in Nude Mice,Experiment of Diaphragmatic Stasis Expelling Decoction Reversing MDR-1 Expression#br# in Transplantation Tumor in Nude Mice,Bax Expression and H460 Apoptosis Induced by Recombined Plasmid pGL3/EGR1-Bax Combined with Radiotherapy in Lung Cancer,Effect of PPARγ Ligands 15d-PGJ2 on Gastric Cancer Growth and Its Mechanism in vivo,Rapamycin in Growth Inhibition of Human Cervical Carcinoma HeLa Cell Subcutaneous#br# Xenografts in Nude Mice and Its Mechanism,Curcumin Inhibits Human Gastric Cancer Growth in Xenograft Murine Model through Downregulation of HIF-1α/MMP-9 Pathway,Effect of RNA Interference Combined with Vincristine on U937 Cells Growth in vitro and in vivo,Inhibiting Effect of Scorpid Poison Combined with Paclitaxe on Human Gastric Cancer MKN-45 Transplanted in Nude Mouse,Therapeutic Efficacy of VEGFR and EGFR Inhibitor Combined with Radiotherapy for Squamous Cell Carcinoma in Nude Mice,Study on VP3 Gene Therapy for Murine Transplanted Hepatocarcinoma Delivered by UTMD and PEI-Gal,Effects of Cdx2 Overexpression on Growth and Metastasis of Transplanted Tumor of Human Gastric Cancer in Nude Mice
Abstract:
摘要 目的 探讨pCMV AIM-1 antisence重组质粒对卵巢癌裸鼠移植瘤的体内抑瘤效应。方法 （1）人卵巢癌细胞A2780转染pCMV空载体和pCMV AIM-1 antisense重组质粒，G418筛选出抗性克隆，扩增后产生的稳定转染株用于实验；（2）通过RT-PCR、Western blot检测未转染A2780细胞（对照组）、A2780转染pCMV空载体和pCMV AIM-1 antisense重组质粒三组AIM-1 mRNA和蛋白的表达；（3）将稳定转染的pCMV空载体和pCMV AIM-1 antisense重组质粒的A2780细胞注入裸鼠皮下，建立裸鼠移植瘤模型，未转染A2780细胞注入裸鼠皮下作为对照，观察三组裸鼠移植瘤生长情况，计算肿瘤重量和肿瘤体积，并进行统计对比分析；（4）SP免疫组织化学法检测移植瘤组织切片AIM-1的表达。结果 （1）A2780对照组转染pCMV空载体组AIM-1 mRNA和蛋白表达无明显改变；而A2780转染pCMV AIM-1 antisense重组质粒组细胞AIM-1 mRNA和蛋白表达明显下降；（2）A2780对照组、A2780转染pCMV空载体组和A2780转染pCMV AIM-1 antisense重组质粒组的裸鼠肿瘤重量分别为（1.93±0.21）g、（1.90±0.39）g和(0.77±0.34）g；移植瘤体积分别为（1 011.7±235.6）mm3、（981.2±162.3）mm3和（468.7±119.6）mm3，重组质粒组与空载体组和对照组相比，差异有统计学意义（P<0.01）；pCMV AIM-1 antisense重组质粒的抑瘤率达53.8%。（3）SP免疫组织化学法检测瘤体组织切片AIM-1的表达情况，见A2780对照组和转染pCMV空载体组AIM-1表达没有明显改变，而A2780转染pCMV AIM-1antisense重组质粒组AIM-1的表达明显减少。重组质粒组AIM-1蛋白表达阳性率和阳性标记指数均明显低于对照组和空载体组（P<0.01）。 结论 pCMV AIM-1 antisense重组质粒对A2780移植瘤生长有明显抑制作用
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