%0 Journal Article
%T 重组质粒pCMV AIM-1 antisense对卵巢癌裸鼠移植瘤的抑瘤效应研究
%A 余涛
%A 刘礼
%A 吕秀玮
%A 袁昌劲
%A 贺文煜
%A 郭敬杰
%A 陈燕浩
%J 肿瘤防治研究
%D 2015
%R 10.3971/j.issn.1000-8578.2015.11.006
%X 摘要 目的 探讨pCMV AIM-1 antisence重组质粒对卵巢癌裸鼠移植瘤的体内抑瘤效应。方法 （1）人卵巢癌细胞A2780转染pCMV空载体和pCMV AIM-1 antisense重组质粒，G418筛选出抗性克隆，扩增后产生的稳定转染株用于实验；（2）通过RT-PCR、Western blot检测未转染A2780细胞（对照组）、A2780转染pCMV空载体和pCMV AIM-1 antisense重组质粒三组AIM-1 mRNA和蛋白的表达；（3）将稳定转染的pCMV空载体和pCMV AIM-1 antisense重组质粒的A2780细胞注入裸鼠皮下，建立裸鼠移植瘤模型，未转染A2780细胞注入裸鼠皮下作为对照，观察三组裸鼠移植瘤生长情况，计算肿瘤重量和肿瘤体积，并进行统计对比分析；（4）SP免疫组织化学法检测移植瘤组织切片AIM-1的表达。结果 （1）A2780对照组转染pCMV空载体组AIM-1 mRNA和蛋白表达无明显改变；而A2780转染pCMV AIM-1 antisense重组质粒组细胞AIM-1 mRNA和蛋白表达明显下降；（2）A2780对照组、A2780转染pCMV空载体组和A2780转染pCMV AIM-1 antisense重组质粒组的裸鼠肿瘤重量分别为（1.93±0.21）g、（1.90±0.39）g和(0.77±0.34）g；移植瘤体积分别为（1 011.7±235.6）mm3、（981.2±162.3）mm3和（468.7±119.6）mm3，重组质粒组与空载体组和对照组相比，差异有统计学意义（P<0.01）；pCMV AIM-1 antisense重组质粒的抑瘤率达53.8%。（3）SP免疫组织化学法检测瘤体组织切片AIM-1的表达情况，见A2780对照组和转染pCMV空载体组AIM-1表达没有明显改变，而A2780转染pCMV AIM-1antisense重组质粒组AIM-1的表达明显减少。重组质粒组AIM-1蛋白表达阳性率和阳性标记指数均明显低于对照组和空载体组（P<0.01）。 结论 pCMV AIM-1 antisense重组质粒对A2780移植瘤生长有明显抑制作用
%K 重组质粒
%K AIM-1
%K 移植瘤
%K 裸鼠
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%U http://www.zlfzyj.com/CN/abstract/abstract8629.shtml