原代培养人喉鳞癌细胞中SIX1、TGF-β、VEGF-C表达的相关性
DOI: 10.3971/j.issn.1000-8578.2016.06.004
Keywords: SIX1,TGF-&beta,VEGF-C,原代人喉鳞癌细胞,Effect of Hypoxia on Expression of HIF-1α, GLUT-1 and MMP-2 in Laryngeal Carcinoma Cell Line Hep-2,In vitro Investigation of Antitumor Efficacy of DpC on Head and Neck Cancer,Surgical Operation and Perioperative Management for Elderly Hypopharyngeal Cancer Patients,Relationship between Local Recurrence and Anterior Commissure Involvement in Early Glottic Laryngocarcinoma after Radiofrequency Coblation,Clinical Efficacy and Prognosis of Concomitant Chemo-radiotherapy and Simple Radiotherapy on Patients with Advanced Laryngeal Cancer after Laryngectomy,Association between Brg1 Single Nucleotide Polymorphism and Laryngo Carcinoma,Anti-laryngocarcinoma Immunity of DC Vaccine which Silenced socs1 by siRNA,Expression and Significance of EGFR and TSLC1 in Different Laryngeal Lesions,Influence of Anti-apoptosis Induced by Recombinant survivin Adenovirus in Laryngeal Cacinoma Cells,Effects of Nimesulide on Expression of CD44 and MMP-7 in Nude Mice Transplanted Hep-2 Cell Line of Human Laryngeal Squamous Cell Carcinoma,Expression of MUC18 and Its Correlation with Counts of Microvessel and Lymph Vessel in Laryngeal Squamous Cell Carcinoma Tissues,Three Human Carcinoma Cell Lines with Different Sensitivity to Adenovirus Expressed E1A Gene,Effect of Simultaneously Blocking VEGF,hTERT and Bcl-xl Expression on Laryngeal Squamous Carcinoma
Abstract:
摘要 目的 探讨原代人喉鳞癌细胞中SIX1、TGF-β、VEGF-C表达相关性。方法 取新鲜人喉鳞状细胞癌组织，进行原代细胞培养，利用基因干扰技术，制备SIX1、TGF-β及SIX1+TGF-β靶向siRNA 转染至人喉鳞癌细胞，转染成功后将实验细胞分为5组： A组（未转染组）、B组（转染空载体组）、C组（SIX1-siRNA组）、D组（TGFβ-siRNA组）、E组（SIX1+TGF-β-siRNA组）。Western blot及细胞爬片免疫荧光检测各组细胞SIX1、TGF-β及VEGF-C蛋白的表达。RT-PCR检测各组细胞SIX1、TGF-β及VEGF-C mRNA的表达。结果 SIX1、TGF-β、VEGF-C蛋白及其mRNA的表达在未转染组、转染空载体组中差异无统计学意义。同未转染组相比，SIX1蛋白及其mRNA的表达在SIX1-siRNA组降低的同时出现了VEGF-C表达的降低；TGF-β蛋白及其mRNA的表达在TGFβ-siRNA组降低的同时也出现了VEGF-C表达的降低，差异均有统计学意义。同SIX1-siRNA组、 TGFβ-siRNA组相比，VEGF-C蛋白及其mRNA的表达在SIX1+TGF-β-siRNA组未出现进一步降低。结论 SIX1和TGF-β能够共同作用促进VEGF-C蛋白及其 mRNA的表达，进而促进人喉鳞癌的淋巴转移。SIX1、TGF-β的变化可能成为临床抑制人喉鳞癌淋巴结转移的潜在靶点
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