%0 Journal Article
%T 原代培养人喉鳞癌细胞中SIX1、TGF-β、VEGF-C表达的相关性
%A 张继华
%A 李丹
%A 王来
%A 苏静
%A 陈春菊
%A 韩彩莉
%J 肿瘤防治研究
%D 2016
%R 10.3971/j.issn.1000-8578.2016.06.004
%X 摘要 目的 探讨原代人喉鳞癌细胞中SIX1、TGF-β、VEGF-C表达相关性。方法 取新鲜人喉鳞状细胞癌组织，进行原代细胞培养，利用基因干扰技术，制备SIX1、TGF-β及SIX1+TGF-β靶向siRNA 转染至人喉鳞癌细胞，转染成功后将实验细胞分为5组： A组（未转染组）、B组（转染空载体组）、C组（SIX1-siRNA组）、D组（TGFβ-siRNA组）、E组（SIX1+TGF-β-siRNA组）。Western blot及细胞爬片免疫荧光检测各组细胞SIX1、TGF-β及VEGF-C蛋白的表达。RT-PCR检测各组细胞SIX1、TGF-β及VEGF-C mRNA的表达。结果 SIX1、TGF-β、VEGF-C蛋白及其mRNA的表达在未转染组、转染空载体组中差异无统计学意义。同未转染组相比，SIX1蛋白及其mRNA的表达在SIX1-siRNA组降低的同时出现了VEGF-C表达的降低；TGF-β蛋白及其mRNA的表达在TGFβ-siRNA组降低的同时也出现了VEGF-C表达的降低，差异均有统计学意义。同SIX1-siRNA组、 TGFβ-siRNA组相比，VEGF-C蛋白及其mRNA的表达在SIX1+TGF-β-siRNA组未出现进一步降低。结论 SIX1和TGF-β能够共同作用促进VEGF-C蛋白及其 mRNA的表达，进而促进人喉鳞癌的淋巴转移。SIX1、TGF-β的变化可能成为临床抑制人喉鳞癌淋巴结转移的潜在靶点
%K SIX1
%K TGF-&beta
%K VEGF-C
%K 原代人喉鳞癌细胞
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%U http://www.zlfzyj.com/CN/abstract/abstract8766.shtml