布洛芬通过PI3K/Akt/mTOR信号通路调控肝癌QGY-7703细胞迁移和侵袭的机制
DOI: 10.3971/j.issn.1000-8578.2016.12.005
Keywords: A Meta-analysis,Meta-analysis of Efficacy and Safety of Jinlong Capsule Combined with Chemoradiotherapy on Liver Metastases,Effects of Sanguinarine on Invasion, Migration and Wnt/β-catenin Signaling Pathway of Lung Adenocarcinoma Cells,Expression and Functional of LncRNA XIST in MCF-7 Breast Cancer Cells,Effect of Down-regulation of HOXB7 Expression on Proliferation, Migration and Invasion Abilities of Hepatoma Cells,Clinicopathologic Characteristics and Prognosis of De Novo Metastatic Breast Cancer Patients,Bone Metastasis in Children with Metanephric Adenosarcoma: A Case Report and Literature Review#br#,Effects of miR-144 on Proliferation, Migration, Invasion and PI3K Pathway of Pancreatic Cancer SW1990 Cells,G6PD对大肠癌细胞生长侵袭的影响及其与HKⅡ的相关性,Effects of MicroRNA-4465 on Apoptosis and Invasion of Colon Cancer Cells,Risk Factors of Pathological Upstaging for Patients with Clinical T2N0M0 Esophageal Squamous Cell Carcinoma,Efficacy and Prognostic Factors of Docetaxel Rechallenge on Metastatic Castrationresistant Prostate Cancer Patients,Advances in Molecular Mechanisms of Early Bone Metastasis,Effect of FBXL20 on Proliferation, Migration and Invasion of Gastric Cancer Cells
Abstract:
摘要 目的 探讨布洛芬通过PI3K/Akt/mTOR信号通路对肝癌QGY-7703细胞迁移和侵袭的调控机制。方法 取对数生长期人肝癌细胞QGY-7703，采用随机法分为两组，对照组（C组）：加入等容量的RPMI l640培养液；实验组（B组）：按照不同布洛芬浓度分为三个亚组：B1组250 μmol/L，B2组500 μmol/L、B3组1 000 μmol/L。各组分别作用24、48和72 h后，利用Transwell小室检测各组细胞的侵袭和迁移能力。各组分别作用48 h后，采用Real-time PCR检测各组细胞中PI3K、PTEN和MMP-9基因表达的变化；用Western blot检测各组细胞中PTEN、Akt、磷酸化Akt（p-Akt）、mTOR、磷酸化mTOR（p-mTOR）和MMP-9蛋白表达情况。结果 与C组比较，B1、B2、B3三组细胞迁移和侵袭能力均降低，具有浓度和时间的依赖性，差异有统计学意义（P<0.01）；与C组比较，B1、B2、B3三组细胞PTEN mRNA和PTEN蛋白表达明显升高，且随着布洛芬作用浓度的增加而升高，具有浓度依赖性，差异有统计学意义（P<0.05）；与C组比较，B1、B2、B3三组细胞PI3K mRNA、Akt和mTOR蛋白的表达量差异均无统计学意义（P>0.05）；与C组比较，B1、B2、B3三组细胞MMP-9 mRNA和蛋白的表达以及p-Akt、p-mTOR蛋白的表达均显著下降，且随着布洛芬作用浓度的增加而降低，具有良好的浓度依赖性，差异有统计学意义（P<0.05）。结论 布洛芬可以抑制QGY-7703细胞的迁移和侵袭能力，与布洛芬对细胞PI3K/Akt/mTOR信号通路的调控有关
Full-Text
