%0 Journal Article
%T 布洛芬通过PI3K/Akt/mTOR信号通路调控肝癌QGY-7703细胞迁移和侵袭的机制
%A 南振华
%A 潘灵辉
%J 肿瘤防治研究
%D 2016
%R 10.3971/j.issn.1000-8578.2016.12.005
%X 摘要 目的 探讨布洛芬通过PI3K/Akt/mTOR信号通路对肝癌QGY-7703细胞迁移和侵袭的调控机制。方法 取对数生长期人肝癌细胞QGY-7703，采用随机法分为两组，对照组（C组）：加入等容量的RPMI l640培养液；实验组（B组）：按照不同布洛芬浓度分为三个亚组：B1组250 μmol/L，B2组500 μmol/L、B3组1 000 μmol/L。各组分别作用24、48和72 h后，利用Transwell小室检测各组细胞的侵袭和迁移能力。各组分别作用48 h后，采用Real-time PCR检测各组细胞中PI3K、PTEN和MMP-9基因表达的变化；用Western blot检测各组细胞中PTEN、Akt、磷酸化Akt（p-Akt）、mTOR、磷酸化mTOR（p-mTOR）和MMP-9蛋白表达情况。结果 与C组比较，B1、B2、B3三组细胞迁移和侵袭能力均降低，具有浓度和时间的依赖性，差异有统计学意义（P<0.01）；与C组比较，B1、B2、B3三组细胞PTEN mRNA和PTEN蛋白表达明显升高，且随着布洛芬作用浓度的增加而升高，具有浓度依赖性，差异有统计学意义（P<0.05）；与C组比较，B1、B2、B3三组细胞PI3K mRNA、Akt和mTOR蛋白的表达量差异均无统计学意义（P>0.05）；与C组比较，B1、B2、B3三组细胞MMP-9 mRNA和蛋白的表达以及p-Akt、p-mTOR蛋白的表达均显著下降，且随着布洛芬作用浓度的增加而降低，具有良好的浓度依赖性，差异有统计学意义（P<0.05）。结论 布洛芬可以抑制QGY-7703细胞的迁移和侵袭能力，与布洛芬对细胞PI3K/Akt/mTOR信号通路的调控有关
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%U http://www.zlfzyj.com/CN/abstract/abstract8887.shtml