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DOI: 10.3971/j.issn.1000-8578.2015.02.006
Keywords: A Meta-analysis,UGCG is Involved in Oxaliplatin Resistance Mechanism of Human Colon Cancer Through Regulating MDR1/P-gp Expression,Chemotherapy Promotes Mesenchymal Stem Cells Homing by Enhancing Cytokine Receptors Expression on Tumor Cells,Effect of GANT61 on Apoptosis of Esophageal Squamous Carcinoma Cells and Its Mechanism,Experiment of Different Administration Sequences of Apatinib and Paclitaxel on Lung Cancer,Clinical Investigation on Qingfei Mixture Combined with Chemotherapy on Middle and Advanced Non-small Cell Lung Cancer,Predictive Factors for Customizing Chemotherapy on Advanced Lung Adenocarcinoma,Bioinformatics Analysis of Genes Related to Multidrug Resistance in Ovarian Cancer
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摘要 目的 探讨沉默stathmin基因后，食管鳞癌细胞对紫杉醇敏感度的改变。方法 利用RNA干扰技术特异性沉默食管鳞癌KYSE150细胞的stathmin基因；实时定量PCR及蛋白质印迹法检测stathmin表达变化；CCK-8检测紫杉醇敏感度变化；流式细胞仪检测细胞周期变化；细胞分裂指数实验观察细胞有丝分裂改变。结果 特异性转染后，stathmin基因mRNA 和蛋白表达均受抑制。沉默stathmin基因后，KYSE150细胞对紫杉醇敏感度增强191.4倍；紫杉醇对特异性转染和未转染的KYSE150细胞半数抑制浓度分别为0.018 nM和3.445 nM。经紫杉醇（0.01 nM）处理72 h后，相比于未转染细胞，特异性转染的KYSE150细胞出现明显G2/M周期阻滞[（55.4±6.2%）vs.（19.1±2.7%）, P=0.000]， 有丝分裂指数减少[（5.6±0.8%）vs.（13.5±3.7%）, P=0.000]。结论 stathmin基因沉默通过细胞周期G2/M阻滞增强食管鳞癌细胞对紫杉醇的化疗敏感度
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